Journal: Cells

Article Title: Cardiac Remodeling in the Absence of Cardiac Contractile Dysfunction Is Sufficient to Promote Cancer Progression

doi: 10.3390/cells11071108

Figure Lengend Snippet: The tumor promotion phenotype is mediated by secreted factors, expressed in either the heart or the tumor. ( A ) Serum levels of periostin, fibronectin and CTGF were determined via ELISA. ( B , C ) mRNA levels from either the heart ( B ) or tumor ( C ) of the indicated genes were measured by means of qRT-PCR, normalized with GAPDH (heart) or Hsp90 (tumor) house-keeping genes. Data are presented as relative expression compared with control, determined as 1. Data are presented as mean ± SEM and were analyzed via multiple Student’s t -tests. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: The quantification of periostin, fibronectin and connective tissue growth factor (CTGF) in the serum was performed using the Mouse Periostin/OSF-2 Quantikine ELISA Kit (R&D systems Inc., Minneapolis, MN, USA), Mouse Fibronectin ELISA Kit (E-EL-M0506, Elabscience, and Mouse CTGF-connective tissue growth factor ELISA Kit (E-EL-M0340, Elabscience, Houston, TX, USA), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: Journal of pharmacological sciences

Article Title: Oligo-peptide I-C-F-6 inhibits hepatic stellate cell activation and ameliorates CCl 4 -induced liver fibrosis by suppressing NF-κB signaling and Wnt/β-catenin signaling.

doi: 10.1016/j.jphs.2018.01.003

Figure Lengend Snippet: Fig. 3. The effects of oligo-peptide I-C-F-6 on HSC activation markers and cytokine levels. (A) The immunohistochemistry of TGF-b1 and a-SMA. Magnification, 200. (B) The effects of oligo-peptide I-C-F-6 on TGF-b1 and a-SMA levels in vitro. The data are expressed as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared with 0 mg/ mL. (C) The effects of oligo-peptide I-C-F-6 on TGF-b1 and a-SMA levels in vivo. (DeG) Serum levels of CTGF, TNF-a, VEGF, TGF-b1. Data are expressed as means ± SD (n ¼ 6e8). #P < 0.05, ##P < 0.01 compared with the control group; *P < 0.05, **P < 0.01 compared with the CCl4 group.

Article Snippet: The MMP-2, MMP-9, TIPM-1, CTGF, TGF-b1, TNF-a, and VEGF ELISA kits were purchased from Cusabio Biotech (Wuhan, China).

Techniques: Activation Assay, Immunohistochemistry, In Vitro, In Vivo, Control

Journal: Molecular Medicine Reports

Article Title: Molecular responses of radiation-induced liver damage in rats

doi: 10.3892/mmr.2014.3051

Figure Lengend Snippet: Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and Smad7 staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.

Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

Techniques: Immunohistochemical staining, Staining, Irradiation

Journal: Molecular Medicine Reports

Article Title: Molecular responses of radiation-induced liver damage in rats

doi: 10.3892/mmr.2014.3051

Figure Lengend Snippet: Western blot analysis (ratio of the molecules investigated, vs. GAPDH). Similar to the results obtained using the reverse transcription quantitative polymerase chain reaction, the protein expression levels were as follows: NF-κB p65 was increased between 3 days and 12 weeks after irradiation. CTGF and Smad3 were significantly increased between 2 and 12 weeks after irradiation. TGF-β1 was significantly increased between 1 and 12 weeks after irradiation and TNF-α was significantly increased between 3 days and 4 weeks after irradiation. Smad4 was significantly increased between 3 days and 1 week after irradiation. Smad7 was significantly increased 3 days after irradiation and reduced significantly 1 and 2 weeks after irradiation, however, it remained higher than that in the control. From 4 weeks post-irradiation, the protein expression of Smad7 returned to the control level. * P<0.05, ** P<0.001 compared with the control group (0 Gy). Gy, grays; CTGF, connective tissue growth factor; TGF-β1, transforming growth factor-β1; TNFα, tumor necrosis factor-α; Smad, mothers against decapentaplegic; NF, nuclear factor.

Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

Techniques: Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Irradiation, Control

Journal: Molecular Medicine Reports

Article Title: Molecular responses of radiation-induced liver damage in rats

doi: 10.3892/mmr.2014.3051

Figure Lengend Snippet: mRNA expression levels were determined using reverse transcription quantitative polymerase chain reaction (ratio of the molecules investigated, vs. GAPDH). The mRNA expression of NF-κB p65 was upregulated between 3 days and 12 weeks after irradiation. The mRNA expression levels were as follows: CTGF and Smad3 were significantly upregulated between 2 and 12 weeks after irradiation. TGF-β1 was significantly upregulated between 1 and 12 weeks after irradiation. TNF-α was significantly upregulated between 3 days and 4 weeks after irradiation. Smad4 was significantly upregulated 3 days and 1 week after irradiation. Smad7 was significantly upregulated 3 days after irradiation and reduced significantly 1–2 weeks after irradiation, however, the expression levels remained higher than that in the control. From 4 weeks post-irradiation, the mRNA expression of Smad7 returned to the control level. * P<0.05, ** P<0.001, compared with the control group (0 Gy). CTGF, connective tissue growth factor; TGF-β1, transforming growth factor-β1; TNFα, tumor necrosis factor-α, Smad, mothers against decapentaplegic; NF, nuclear factor.

Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Irradiation, Control